Journal of Applied Hematology

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 13  |  Issue : 3  |  Page : 132--138

Evaluation of sensitivity and specificity of high fluorescence lymphocyte count percentage of sysmex XN analyzer in diagnosis of dengue


Aparna Ningombam1, Shreyam Acharya1, Abhirup Sarkar1, Kundan Kumar1, Megha Brijwal2, Srimantinee Routray1, Janya Sachdev2, Deepankar Srigyan2, Aashish Choudhary2, Arulselvi Subramanian1, Lalit Dar2 
1 Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India
2 Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Megha Brijwal
8005B, Virology, Department of Microbiology, Convergence Block, All India Institute of Medical Sciences, New Delhi - 110 029
India

BACKGROUND: The Sysmex XN-series hematology analyzers provide newer parameters including high fluorescence lymphocyte cell percentage (HFLC%) which correlates with the presence of atypical lymphocytes in peripheral blood. We aimed to analyze the sensitivity and specificity of HFLC% as a diagnostic tool and its association with serological status in diagnosed dengue patients and thereby establish a cutoff of HFLC% based on serology. Besides, we also wish to correlate HFLC% with thrombocytopenia in these patients. MATERIALS AND METHODS: A total of 1500 serum samples were subjected to serological evaluation for dengue. After excluding hematological malignancies and autoimmune disorders, the same day complete blood count parameters including HFLC% and platelet counts were collected retrospectively for 292 serologically positive dengue cases and 76 seronegative controls. RESULTS: Our result shows that in nonstructural 1 antigen-positive cases, a cutoff of >5.2% HFLC can have a sensitivity of 79.5% and specificity of 98.6%. We found a different cut off of HFLC% >3.2% (sensitivity 83.4%, specificity 98.6%) for the cases with only immunoglobulin M positivity and a cut off of HFLC% >2.6% (sensitivity 86.1%, specificity 96%) in the dual positive cases (immunoglobulin M with nonstructural 1 antigen). Besides, high HFLC% also shows a strong correlation with platelet count with a Spearman correlation coefficient of −0.6. CONCLUSIONS: The result of our study shows that a specific cutoff of HFLC% can not only help us to suspect dengue fever but also predict the risk of thrombocytopenia in already diagnosed dengue patients. The sensitivity and specificity of HFLC% varied with the serological status of the patients which depend on the days of fever on presentation.


How to cite this article:
Ningombam A, Acharya S, Sarkar A, Kumar K, Brijwal M, Routray S, Sachdev J, Srigyan D, Choudhary A, Subramanian A, Dar L. Evaluation of sensitivity and specificity of high fluorescence lymphocyte count percentage of sysmex XN analyzer in diagnosis of dengue.J Appl Hematol 2022;13:132-138


How to cite this URL:
Ningombam A, Acharya S, Sarkar A, Kumar K, Brijwal M, Routray S, Sachdev J, Srigyan D, Choudhary A, Subramanian A, Dar L. Evaluation of sensitivity and specificity of high fluorescence lymphocyte count percentage of sysmex XN analyzer in diagnosis of dengue. J Appl Hematol [serial online] 2022 [cited 2022 Sep 25 ];13:132-138
Available from: https://www.jahjournal.org/article.asp?issn=1658-5127;year=2022;volume=13;issue=3;spage=132;epage=138;aulast=Ningombam;type=0