Figure 3: Gap-PCR was performed using 50 ng/μL of DNA samples and was run on 1.2% agarose gels. The 974 bp represents deletion fragment HPFH-6, the 1447 bp represents deletion fragment Thai, the 620 bp represents deletion fragment Siriraj, and 304 bp represents internal control. M represents the 200 bp DNA ladder. bp: Base pair, NTC: Nontemplate control, HPFH-6: Hereditary persistence of fetal hemoglobin type-6. PCR: Polymerase chain reaction